Synthego offers researchers the ability to order single guide RNAs (sgRNAs) for their bench work. Synthego sgRNAs can be ordered with site-specific chemical modification powered by synthego's Halo platform. Synthego also operates a GMP production facility to produce GMP-grade synthetic sgRNA.
Service Offerings:
IPS cells
Immortalized cell lines
CRISPR design tools
Chemically modified multi-guide libraries for CRISPR screening
Vendors for guide RNA will state a purity during negotiations and when they deliver mateirals. Synthego is a great example of why you should not believe these projections. The stated that they had 80%+ purity for custom guide RNAs that were ~100nt in... length. This was becuase their HPLC program was incredibly short. Once we ran the material on a proper HPLC procedure, it was obvious that it was less than 30% pure. It was, by far, the least pure of any guide RNA that we recieved from a CDMO partner. I'd recomend going with Biospring, Agilent, or Sumitomo instead.
